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Permissible peptide insertions surrounding the signal peptide-mature protein junction of the ClpG prepilin: CS31A fimbriae of Escherichia coli as carriers of foreign sequences

The clpG gene, expressing the Escherichia coli major CS31A fimbrial subunit ClpG, was subjected to random mutagenesis by insertion of an EcoRI linker and a kanamycin-resistance (Km(R)) cassette into the multiple newly generated EcoRI sites. The Km(R) gene was then excised by PstI, which left a 48-bp...

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Bibliografische gegevens
Gepubliceerd in:Gene
Hoofdauteurs: Der Vartanian, Maurice, Méchin, Marie-Claire, Jaffeux, Bernard, Bertin, Yolande, Félix, Isabelle, Gaillard-Martinie, Brigitte
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Published by Elsevier B.V. 1994
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7131889/
https://ncbi.nlm.nih.gov/pubmed/7523252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0378-1119(94)90229-1
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