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A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to...

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Detalhes bibliográficos
Publicado no:J Clin Virol
Main Authors: Poon, Leo L.M, Wong, Bonnie W.Y, Chan, Kwok Hung, Leung, Cynthia S.W, Yuen, Kwok Yung, Guan, Yi, Peiris, Joseph S.M
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier B.V. 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7129749/
https://ncbi.nlm.nih.gov/pubmed/15135737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jcv.2003.12.007
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