High-level prokaryotic expression of envelope exterior of membrane protein of porcine epidemic diarrhea virus

The truncated fragment M′ gene, encoding the exterior of the viral envelope protein of PEDV, was subcloned into prokaryotic expression vector pGEX-6p-1. The recombinant plasmid pGEX-6p-M′ was constructed and transformed into E. coli BL21(DE3)pLysS for expression. SDS-PAGE analysis showed recombinant...

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Bibliografiske detaljer
Udgivet i:Vet Microbiol
Main Authors: Shenyang, Gao, Enhui, Zha, Baoxian, Li, Xinyuan, Qiao, Lijie, Tang, Junwei, Ge, Yijing, Li
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier B.V. 2007
Fag:
Short Communication
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7127142/
https://ncbi.nlm.nih.gov/pubmed/17475420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vetmic.2007.03.027
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