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High-level prokaryotic expression of envelope exterior of membrane protein of porcine epidemic diarrhea virus
The truncated fragment M′ gene, encoding the exterior of the viral envelope protein of PEDV, was subcloned into prokaryotic expression vector pGEX-6p-1. The recombinant plasmid pGEX-6p-M′ was constructed and transformed into E. coli BL21(DE3)pLysS for expression. SDS-PAGE analysis showed recombinant...
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| Publicado no: | Vet Microbiol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier B.V.
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7127142/ https://ncbi.nlm.nih.gov/pubmed/17475420 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vetmic.2007.03.027 |
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