Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye

A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid visual detection of turkey coronavirus (TCoV) infection. The reaction is performed in one step in a single tube at 65 °C for 45 min, with hydroxynaphthol blue (HNB) dye added prior to...

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Detalhes bibliográficos
Publicado no:Mol Cell Probes
Main Authors: Cardoso, Tereza C., Ferrari, Heitor F., Bregano, Lívia C., Silva-Frade, Camila, Rosa, Ana Carolina G., Andrade, Alexandre L.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Ltd. 2010
Assuntos:
Short Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7125592/
https://ncbi.nlm.nih.gov/pubmed/20732411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2010.08.003
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