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Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye

BACKGROUND: The aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus. RESULTS: The detection limit of LAMP using in vitro RNA transcripts was 3.6×10 copies·μL(-1), which is as sen...

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Detalhes bibliográficos
Main Authors: Yang, Bo-Yun, Liu, Xiao-Lu, Wei, Yu-Mei, Wang, Jing-Qi, He, Xiao-Qing, Jin, Yi, Wang, Zi-Jian
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3930758/
https://ncbi.nlm.nih.gov/pubmed/24524254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-14-38
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