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Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones
Some DNA fragments are difficult to clone in Escherichia coli by standard methods. It has been speculated that unintended transcription and translation result in expression of proteins that are toxic to the bacteria. This problem is frequently observed during assembly of infectious full-length virus...
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| Veröffentlicht in: | Plant Virology Protocols |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2008
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7120191/ https://ncbi.nlm.nih.gov/pubmed/18370279 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-102-4_36 |
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