Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay

A method was developed for the quantitation of respiratory syncytial virus (RSV) based on real-time RT-PCR using a LightCycler instrument. A control real-time RT-PCR was undertaken on GAPDH mRNA (a human housekeeping gene) was carried out to standardise the non-homogeneous respiratory samples. The r...

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Bibliographische Detailangaben
Veröffentlicht in:J Virol Methods
Hauptverfasser: Gueudin, M, Vabret, A, Petitjean, J, Gouarin, S, Brouard, J, Freymuth, F
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier Science B.V. 2003
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7119593/
https://ncbi.nlm.nih.gov/pubmed/12668266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(03)00042-9
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