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Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay
A method was developed for the quantitation of respiratory syncytial virus (RSV) based on real-time RT-PCR using a LightCycler instrument. A control real-time RT-PCR was undertaken on GAPDH mRNA (a human housekeeping gene) was carried out to standardise the non-homogeneous respiratory samples. The r...
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| 發表在: | J Virol Methods |
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| Main Authors: | , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier Science B.V.
2003
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7119593/ https://ncbi.nlm.nih.gov/pubmed/12668266 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(03)00042-9 |
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