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Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay

A method was developed for the quantitation of respiratory syncytial virus (RSV) based on real-time RT-PCR using a LightCycler instrument. A control real-time RT-PCR was undertaken on GAPDH mRNA (a human housekeeping gene) was carried out to standardise the non-homogeneous respiratory samples. The r...

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Bibliografske podrobnosti
izdano v:J Virol Methods
Main Authors: Gueudin, M, Vabret, A, Petitjean, J, Gouarin, S, Brouard, J, Freymuth, F
Format: Artigo
Jezik:Inglês
Izdano: Elsevier Science B.V. 2003
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7119593/
https://ncbi.nlm.nih.gov/pubmed/12668266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(03)00042-9
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