Development of duplex real-time PCR for detection of two DNA respiratory viruses

A method was developed for the detection and quantitation of HAdV (human adenovirus) and HBoV (human bocavirus) based on a duplex real-time PCR, the AB PCR, using a Smartcycler instrument. A control real-time PCR was carried out on albumin DNA to standardise the non-homogenous respiratory samples. N...

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Publicado en:J Virol Methods
Autores principales: Dina, Julia, Nguyen, Emilie, Gouarin, Stephanie, Petitjean, Joelle, Parienti, Jean-Jacques, Nimal, Delphine, Brouard, Jacques, Freymuth, François, Vabret, Astrid
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier B.V. 2009
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112853/
https://ncbi.nlm.nih.gov/pubmed/19654024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.07.025
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