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Monitoring of HIV-1 envelope-mediated membrane fusion using modified split green fluorescent proteins

A simple, cell-based, membrane fusion assay system that uses split green fluorescent proteins (spGFPs) as an indicator was developed. The attachment of the pleckstrin homology (PH) domain to the N-termini of each spGFP not only localized the reporter signal to the plasma membrane but also helped the...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Wang, Jianqi, Kondo, Naoyuki, Long, Yufei, Iwamoto, Aikichi, Matsuda, Zene
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier B.V. 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112932/
https://ncbi.nlm.nih.gov/pubmed/19559731
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.06.017
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