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A long distance RT-PCR able to amplify the Pestivirus genome
A method to amplify long genomic regions (up to ∼12.3 kb) from pestiviruses in one RT-PCR is described. The difficulty in designing conserved Pestivirus primers for the amplification of genomes from highly divergent isolates simply by means of overlapping segments is demonstrated using new bioinform...
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| Publicado no: | J Virol Methods |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier B.V.
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7112918/ https://ncbi.nlm.nih.gov/pubmed/16497393 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2006.01.005 |
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