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Long Distance Reverse-Transcription PCR

Polymerase chain reaction (PCR) has been applied to the amplification of long DNA fragments from a variety of sources, including genomic, mitochondrial, and viral DNAs (1-5). We have adapted the concept of long PCR technology to reverse-transcription (RT) PCR (6). Here, we describe the parameters cr...

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Detalles Bibliográficos
Publicado en:PCR Cloning Protocols
Autores principales: Thiel, Volker, Herold, Jens, Siddell, Stuart G.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2002
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7120746/
https://ncbi.nlm.nih.gov/pubmed/12494637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1385/1-59259-177-9:059
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