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Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR
A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was u...
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| Publicat a: | J Virol Methods |
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| Autors principals: | , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier B.V.
2007
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7112852/ https://ncbi.nlm.nih.gov/pubmed/17692932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2007.06.017 |
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