Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR

A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was u...

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Bibliographic Details
Published in:J Virol Methods
Main Authors: Elia, Gabriella, Cavalli, Alessandra, Desario, Costantina, Lorusso, Eleonora, Lucente, Maria Stella, Decaro, Nicola, Martella, Vito, Buonavoglia, Canio
Format: Artigo
Language:Inglês
Published: Elsevier B.V. 2007
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112852/
https://ncbi.nlm.nih.gov/pubmed/17692932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2007.06.017
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