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Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR
A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was u...
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| Опубликовано в: : | J Virol Methods |
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| Главные авторы: | , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Elsevier B.V.
2007
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7112852/ https://ncbi.nlm.nih.gov/pubmed/17692932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2007.06.017 |
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