Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay

A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for the detection of bovine coronavirus (BCoV) RNA in clinical samples is described. The assay is based on TaqMan technology, consisting of two primers and one probe labeled with the reporter dye 6-carboxyfluorescein that binds sel...

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Publicat a:J Virol Methods
Autors principals: Decaro, Nicola, Elia, Gabriella, Campolo, Marco, Desario, Costantina, Mari, Viviana, Radogna, Arianna, Colaianni, Maria Loredana, Cirone, Francesco, Tempesta, Maria, Buonavoglia, Canio
Format: Artigo
Idioma:Inglês
Publicat: Elsevier B.V. 2008
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112840/
https://ncbi.nlm.nih.gov/pubmed/18579223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2008.05.016
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