Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay

A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for the detection of bovine coronavirus (BCoV) RNA in clinical samples is described. The assay is based on TaqMan technology, consisting of two primers and one probe labeled with the reporter dye 6-carboxyfluorescein that binds sel...

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Veröffentlicht in:J Virol Methods
Hauptverfasser: Decaro, Nicola, Elia, Gabriella, Campolo, Marco, Desario, Costantina, Mari, Viviana, Radogna, Arianna, Colaianni, Maria Loredana, Cirone, Francesco, Tempesta, Maria, Buonavoglia, Canio
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier B.V. 2008
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112840/
https://ncbi.nlm.nih.gov/pubmed/18579223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2008.05.016
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