Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains

A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2, and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as...

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Bibliographic Details
Published in:J Virol Methods
Main Authors: Losurdo, Michele, Mari, Viviana, Lucente, Maria Stella, Colaianni, Maria Loredana, Padalino, Iolanda, Cavaliere, Nicola, Buonavoglia, Canio, Decaro, Nicola
Format: Artigo
Language:Inglês
Published: Elsevier B.V. 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7113749/
https://ncbi.nlm.nih.gov/pubmed/26300370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2015.08.013
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