Expression, purification, and characterization of SARS coronavirus RNA polymerase

The RNA-dependent RNA polymerase (RdRp) of SARS coronavirus (SARS-CoV) is essential for viral replication and a potential target for anti-SARS drugs. We report here the cloning, expression, and purification of the N-terminal GST-fused SARS-CoV RdRp and its polymerase catalytic domain in Escherichia...

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Detalhes bibliográficos
Publicado no:Virology
Main Authors: Cheng, Ao, Zhang, Wei, Xie, Youhua, Jiang, Weihong, Arnold, Eddy, Sarafianos, Stefan G., Ding, Jianping
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Inc. 2005
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7111802/
https://ncbi.nlm.nih.gov/pubmed/15840516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.virol.2005.02.017
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