Efficient Overproduction of Active Nitrile Hydratase by Coupling Expression Induction and Enzyme Maturation via Programming a Controllable Cobalt-Responsive Gene Circuit

A robust and portable expression system is of great importance in enzyme production, metabolic engineering, and synthetic biology, which maximizes the performance of the engineered system. In this study, a tailor-made cobalt-induced expression system (CIES) was developed for low-cost and eco-friendl...

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Detalhes bibliográficos
Publicado no:Front Bioeng Biotechnol
Main Authors: Han, Laichuang, Cui, Wenjing, Lin, Qiao, Chen, Qiaoqing, Suo, Feiya, Ma, Ke, Wang, Yang, Hao, Wenliang, Cheng, Zhongyi, Zhou, Zhemin
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Bioengineering and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7105576/
https://ncbi.nlm.nih.gov/pubmed/32266230
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.00193
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