Design and Construction of Portable CRISPR-Cpf1-Mediated Genome Editing in Bacillus subtilis 168 Oriented Toward Multiple Utilities

Bacillus subtilis is an important Gram-positive bacterium for industrial biotechnology, which has been widely used to produce diverse high-value added chemicals and industrially and pharmaceutically relevant proteins. Robust and versatile toolkits for genome editing in B. subtilis are highly demandi...

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Detalhes bibliográficos
Publicado no:Front Bioeng Biotechnol
Main Authors: Hao, Wenliang, Suo, Feiya, Lin, Qiao, Chen, Qiaoqing, Zhou, Li, Liu, Zhongmei, Cui, Wenjing, Zhou, Zhemin
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Bioengineering and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7492563/
https://ncbi.nlm.nih.gov/pubmed/32984297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.524676
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