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Strategies to maximize performance in STimulated Emission Depletion (STED) nanoscopy of biological specimens

Super-resolution fluorescence microscopy has become an important catalyst for discovery in the life sciences. In STimulated Emission Depletion (STED) microscopy, a pattern of light drives fluorophores from a signal-emitting on-state to a non-signalling off-state. Only emitters residing in a sub-diff...

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Bibliographic Details
Published in:Methods
Main Authors: Jahr, Wiebke, Velicky, Philipp, Danzl, Johann Georg
Format: Artigo
Language:Inglês
Published: 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7100895/
https://ncbi.nlm.nih.gov/pubmed/31344404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.07.019
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