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Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell
We demonstrate far-field optical imaging with subdiffraction resolution of the endoplasmic reticulum (ER) in the interior of a living mammalian cell. The diffraction barrier is overcome by applying stimulated emission depletion (STED) on a yellow fluorescent protein tag. Imaging individual structura...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
2008
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2538451/ https://ncbi.nlm.nih.gov/pubmed/18796604 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0807705105 |
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