Stimulated Emission Depletion Nanoscopy of Living Cells Using SNAP-Tag Fusion Proteins

We show far-field fluorescence nanoscopy of different structural elements labeled with an organic dye within living mammalian cells. The diffraction barrier limiting far-field light microscopy is outperformed by using stimulated emission depletion. We used the tagging protein hAGT (SNAP-tag), which...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Hein, Birka, Willig, Katrin I., Wurm, Christian A., Westphal, Volker, Jakobs, Stefan, Hell, Stefan W.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: The Biophysical Society 2010
Gaiak:
Spectroscopy, Imaging, and Other Techniques
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2800968/
https://ncbi.nlm.nih.gov/pubmed/20074516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.09.053
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