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Stimulated Emission Depletion Nanoscopy of Living Cells Using SNAP-Tag Fusion Proteins
We show far-field fluorescence nanoscopy of different structural elements labeled with an organic dye within living mammalian cells. The diffraction barrier limiting far-field light microscopy is outperformed by using stimulated emission depletion. We used the tagging protein hAGT (SNAP-tag), which...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2800968/ https://ncbi.nlm.nih.gov/pubmed/20074516 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.09.053 |
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