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Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples

Long RT-PCR (LRP) amplification of RNA templates is sometimes difficult compared to long PCR of DNA templates. Among RNA templates, hepatitis C virus (HCV) represents an excellent example to challenge the potential of LRP technology due to its extensive secondary structures and its difficulty to be...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Res Commun
Main Authors: Fan, Xiaofeng, Xu, Yanjuan, Di Bisceglie, Adrian M.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Inc. 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7092855/
https://ncbi.nlm.nih.gov/pubmed/16793008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2006.06.039
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