Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique

AIM: To characterize enzymatic activity of severe acute respiratory syndrome (SARS) coronavirus (CoV) 3C-like protease (3CL(pro)) and its four site-directed mutants. METHODS: Based on the fluorescence resonance energy transfer (FRET) principle using 5-[(2′-aminoethyl)-amino] naphthelenesulfonic acid...

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Detalhes bibliográficos
Publicado no:Acta Pharmacol Sin
Main Authors: Chen, Shuai, Chen, Li-li, Luo, Hai-bin, Sun, Tao, Chen, Jing, Ye, Fei, Cai, Jian-hua, Shen, Jing-kang, Shen, Xu, Jiang, Hua-liang
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2005
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7091904/
https://ncbi.nlm.nih.gov/pubmed/15659121
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1745-7254.2005.00010.x
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