Expression and purification of SARS coronavirus membrane protein

To construct a recombinant plasmid Pet23a-M, the gene encoding severe acute respiratory syndrome (SARS) coronavirus membrane protein was amplified by RT-PCR and cloned into the expression plasmid Pet23a. Results of restriction endonuclease analysis, PCR detection and DNA sequencing analysis revealed...

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Dades bibliogràfiques
Publicat a:J Huazhong Univ Sci Technolog Med Sci
Autors principals: Wuxing, Dai, Mingjun, Lei, Shaoting, Wu, Zhihao, Chen, Liang, Liang, Hujrong, Pan, Li, Qin, Shitong, Gao, Shishan, Yuan, Renli, Zhang
Format: Artigo
Idioma:Inglês
Publicat: Huazhong University of Science and Technology 2004
Matèries:
Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7089427/
https://ncbi.nlm.nih.gov/pubmed/15641679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/BF02831095
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