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Degenerate primer design to clone the human repertoire of immunoglobulin heavy chain variable regions
Amplifying the variable (Fv or V) regions of immunoglobulins (Ig) has become a challenge in cloning antibody genes for phage display, a technique used to study protein–protein, protein–peptide, and protein–DNA interactions using bacteriophages to connect proteins with the genetic information that en...
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| Veröffentlicht in: | World J Microbiol Biotechnol |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Springer Netherlands
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7089251/ https://ncbi.nlm.nih.gov/pubmed/22806814 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11274-011-0830-3 |
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