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Degenerated primer design to amplify the heavy chain variable region from immunoglobulin cDNA

BACKGROUND: The amplification of variable regions of immunoglobulins has become a major challenge in the cloning of antibody genes, whether from hybridoma cell lines or splenic B cells. Using conventional protocols, the heavy-chain variable region genes often are not amplified successfully from the...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Wang, Ying, Chen, Wei, Li, Xu, Cheng, Bing
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: BioMed Central 2006
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC1780117/
https://ncbi.nlm.nih.gov/pubmed/17217527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-7-S4-S9
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