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Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris
The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome associated coronavirus (SARS-CoV) is vital for SARS-CoV replication and is a promising drug target. Recombinant 3CL(pro) was expressed in Pichia pastoris GS115 as a 42 kDa protein that displayed a K (m) of 15 ± 2 μM with Dabcyl-KTS...
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| Publicado no: | Biotechnol Lett |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Netherlands
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7087583/ https://ncbi.nlm.nih.gov/pubmed/22350287 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-011-0845-8 |
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