An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins

Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determini...

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Udgivet i:J Biol Methods
Main Authors: Roberts, Michael D., Young, Kaelin C., Fox, Carlton D., Vann, Christopher G., Roberson, Paul A., Osburn, Shelby C., Moore, Johnathon H., Mumford, Petey W., Romero, Matthew A., Beck, Darren T., Haun, Cody T., Badisa, Veera L.D., Mwashote, Benjamin M., Ibeanusi, Victor, Kavazis, Andreas N.
Format: Artigo
Sprog:Inglês
Udgivet: Journal of Biological Methods 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7081056/
https://ncbi.nlm.nih.gov/pubmed/32201709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2020.307
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