An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins

Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determini...

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Vydáno v:J Biol Methods
Hlavní autoři: Roberts, Michael D., Young, Kaelin C., Fox, Carlton D., Vann, Christopher G., Roberson, Paul A., Osburn, Shelby C., Moore, Johnathon H., Mumford, Petey W., Romero, Matthew A., Beck, Darren T., Haun, Cody T., Badisa, Veera L.D., Mwashote, Benjamin M., Ibeanusi, Victor, Kavazis, Andreas N.
Médium: Artigo
Jazyk:Inglês
Vydáno: Journal of Biological Methods 2020
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7081056/
https://ncbi.nlm.nih.gov/pubmed/32201709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2020.307
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