Llwytho...

An in silico and in vitro pipeline for the rapid screening of helicase modulators

To evaluate the potency of potential helicase modulators, we developed an assay of helicase enzyme activity. Using a DNA or RNA biotin labelled oligonucleotide and after the addition of a recombinant helicase, the nucleic acid unwinds, causing the emission of luminescence, which is quantified with a...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:EMBnet J
Prif Awduron: Papakonstantinou, Eleni, Bacopoulou, Flora, Megalooikonomou, Vasileios, Efthimiadou, Aspasia, Vlachakis, Dimitrios
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7079759/
https://ncbi.nlm.nih.gov/pubmed/32190583
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14806/ej.25.0.927
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