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An in silico and in vitro pipeline for the rapid screening of helicase modulators
To evaluate the potency of potential helicase modulators, we developed an assay of helicase enzyme activity. Using a DNA or RNA biotin labelled oligonucleotide and after the addition of a recombinant helicase, the nucleic acid unwinds, causing the emission of luminescence, which is quantified with a...
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| Publicat a: | EMBnet J |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7079759/ https://ncbi.nlm.nih.gov/pubmed/32190583 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14806/ej.25.0.927 |
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