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Dynamic Genome Editing Using In Vivo Synthesized Donor ssDNA in Escherichia coli

As a key element of genome editing, donor DNA introduces the desired exogenous sequence while working with other crucial machinery such as CRISPR-Cas or recombinases. However, current methods for the delivery of donor DNA into cells are both inefficient and complicated. Here, we developed a new meth...

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Detalhes bibliográficos
Publicado no:Cells
Main Authors: Hao, Min, Wang, Zhaoguan, Qiao, Hongyan, Yin, Peng, Qiao, Jianjun, Qi, Hao
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7072734/
https://ncbi.nlm.nih.gov/pubmed/32085579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9020467
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