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An amber obligate active site-directed ligand evolution technique for phage display

Although noncanonical amino acids (ncAAs) were first incorporated into phage libraries through amber suppression nearly two decades ago, their application for use in drug discovery has been limited due to inherent library bias towards sense-containing phages. Here, we report a technique based on sup...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Tharp, Jeffery M., Hampton, J. Trae, Reed, Catrina A., Ehnbom, Andreas, Chen, Peng-Hsun Chase, Morse, Jared S., Kurra, Yadagirri, Pérez, Lisa M., Xu, Shiqing, Liu, Wenshe Ray
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7070036/
https://ncbi.nlm.nih.gov/pubmed/32170178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-15057-7
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