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An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery

Motor neurons form specialized synapses with skeletal muscle fibers, called neuromuscular junctions (NMJs). Cultured myotubes are used as a simplified in vitro system to study the postsynaptic specialization of muscles. The stimulation of myotubes with the glycoprotein agrin or laminin-111 induces t...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Pęziński, Marcin, Daszczuk, Patrycja, Pradhan, Bhola Shankar, Lochmüller, Hanns, Prószyński, Tomasz J.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7066178/
https://ncbi.nlm.nih.gov/pubmed/32161296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-61347-x
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