Lightsheet fluorescence lifetime imaging microscopy with wide‐field time‐correlated single photon counting

We report on wide‐field time‐correlated single photon counting (TCSPC)‐based fluorescence lifetime imaging microscopy (FLIM) with lightsheet illumination. A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single‐photon sensitive camera, is u...

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Détails bibliographiques
Publié dans:J Biophotonics
Auteurs principaux: Hirvonen, Liisa M., Nedbal, Jakub, Almutairi, Norah, Phillips, Thomas A., Becker, Wolfgang, Conneely, Thomas, Milnes, James, Cox, Susan, Stürzenbaum, Stephen, Suhling, Klaus
Format: Artigo
Langue:Inglês
Publié: WILEY‐VCH Verlag GmbH & Co. KGaA 2019
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7065631/
https://ncbi.nlm.nih.gov/pubmed/31661595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jbio.201960099
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