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Analyzing the Functionality of Non-native Hsp70 Proteins in Saccharomyces cerevisiae
Yeast are an ideal system to study Heat Shock Protein 70 (Hsp70) function in a cellular context. This protocol was generated to analyze the function of non-native Hsp70 proteins by expressing them as the sole cytosolic Hsp70 in yeast. As an initial step, Hsp70 variants (such as Ssa1 point mutants an...
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| Gepubliceerd in: | Bio Protoc |
|---|---|
| Hoofdauteurs: | , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Bio-Protocol
2019
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7062368/ https://ncbi.nlm.nih.gov/pubmed/32154330 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3389 |
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