CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures

Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the 5′ triphosphate. The cap guanosine and initial transcribed nucleotides are further methylated by a series of cap methyltransferases to generate the mature cap structures which protect RNA from degradation...

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Dettagli Bibliografici
Pubblicato in:Open Biol
Autori principali: Galloway, Alison, Atrih, Abdelmadjid, Grzela, Renata, Darzynkiewicz, Edward, Ferguson, Michael A. J., Cowling, Victoria H.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Royal Society 2020
Soggetti:
Methods and Techniques
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7058934/
https://ncbi.nlm.nih.gov/pubmed/32097574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsob.190306
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