CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures

Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the 5′ triphosphate. The cap guanosine and initial transcribed nucleotides are further methylated by a series of cap methyltransferases to generate the mature cap structures which protect RNA from degradation...

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Publicat a:Open Biol
Autors principals: Galloway, Alison, Atrih, Abdelmadjid, Grzela, Renata, Darzynkiewicz, Edward, Ferguson, Michael A. J., Cowling, Victoria H.
Format: Artigo
Idioma:Inglês
Publicat: The Royal Society 2020
Matèries:
Methods and Techniques
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7058934/
https://ncbi.nlm.nih.gov/pubmed/32097574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsob.190306
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