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The Two-Species Model of transketolase explains donor substrate-binding, inhibition and heat-activation

We recently characterised a low-activity form of E. coli transketolase, TK(low), which also binds the cofactor thiamine pyrophosphate (TPP) with an affinity up to two-orders of magnitude lower than the previously known high TPP-affinity and high-activity form, TK(high), in the presence of Mg(2+). We...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wilkinson, Henry C., Dalby, Paul A.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7057962/
https://ncbi.nlm.nih.gov/pubmed/32139871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-61175-z
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