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Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Dictyostelium discoideum is an intriguing model organism for the study of cell differentiation processes during development, cell signaling, and other important cellular biology questions. The technologies available to genetically manipulate Dictyostelium cells are well-developed. Transfections can...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Paschke, Peggy, Knecht, David A., Williams, Thomas D., Thomason, Peter A., Insall, Robert H., Chubb, Jonathan R., Kay, Robert R., Veltman, Douwe M.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7039707/
https://ncbi.nlm.nih.gov/pubmed/30735174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58981
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