Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum

Dictyostelium has a mature technology for molecular-genetic manipulation based around transfection using several different selectable markers, marker re-cycling, homologous recombination and insertional mutagenesis, all supported by a well-annotated genome. However this technology is optimized for m...

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Publicat a:PLoS One
Autors principals: Paschke, Peggy, Knecht, David A., Silale, Augustinas, Traynor, David, Williams, Thomas D., Thomason, Peter A., Insall, Robert H., Chubb, Jonathan R., Kay, Robert R., Veltman, Douwe M.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2018
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5976153/
https://ncbi.nlm.nih.gov/pubmed/29847546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196809
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