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Adapted formaldehyde gradient cross-linking protocol implicates human eIF3d and eIF3c, k and l subunits in the 43S and 48S pre-initiation complex assembly, respectively

One of the key roles of the 12-subunit eukaryotic translation initiation factor 3 (eIF3) is to promote the formation of the 43S and 48S pre-initiation complexes (PICs). However, particular contributions of its individual subunits to these two critical initiation reactions remained obscure. Here, we...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Herrmannová, Anna, Prilepskaja, Terezie, Wagner, Susan, Šikrová, Darina, Zeman, Jakub, Poncová, Kristýna, Valášek, Leoš Shivaya
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7039009/
https://ncbi.nlm.nih.gov/pubmed/31863585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1185
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