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Isolation and culture of oculomotor, trochlear, and spinal motor neurons from prenatal Isl(MN):GFP transgenic mice

Oculomotor neurons (CN3s) and trochlear neurons (CN4s) exhibit remarkable resistance to degenerative motor neuron diseases such as amyotrophic lateral sclerosis (ALS) when compared to spinal motor neurons (SMNs). The ability to isolate and culture primary mouse CN3s, CN4s and SMNs would provide an a...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Fujiki, Ryosuke, Lee, Joun Y., Jurgens, Julie A., Whitman, Mary C., Engle, Elizabeth C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7036286/
https://ncbi.nlm.nih.gov/pubmed/31789317
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60440
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