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Optogenetics: Rho GTPases Activated by Light in Living Macrophages

Genetically encoded optogenetic tools are increasingly popular and useful for perturbing signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic procedures employed to implement optogenetics of Rho GTPases in a macrophage cell line. Methods described here ar...

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Bibliografski detalji
Izdano u:Methods Mol Biol
Glavni autori: Hülsemann, Maren, Verkhusha, Polina V., Guo, Peng, Miskolci, Veronika, Cox, Dianne, Hodgson, Louis
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7032845/
https://ncbi.nlm.nih.gov/pubmed/31939189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0247-8_24
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