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A Mix-and-Measure Assay for Determining the Activation Status of Endogenous Cdc42 in Cytokine-stimulated Macrophage Cell Lysates

Cytokine stimulations of leukocytes many times result in transient activation of the p21 Rho family of small GTPases. The role of these molecules during cell migration and chemotaxis is well established. The traditional approach to study the activation dynamics of these proteins involves affinity pu...

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Bibliographic Details
Main Authors: Miskolci, Veronika, Spiering, Désirée, Cox, Dianne, Hodgson, Louis
Format: Artigo
Language:Inglês
Published: 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4133094/
https://ncbi.nlm.nih.gov/pubmed/24908304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-0928-5_15
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