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Ultra-high throughput detection of single cell β-galactosidase activity in droplets using micro-optical lens array

We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli β-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their...

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Dades bibliogràfiques
Publicat a:Appl Phys Lett
Autors principals: Lim, Jiseok, Vrignon, Jérémy, Gruner, Philipp, Karamitros, Christos S., Konrad, Manfred, Baret, Jean-Christophe
Format: Artigo
Idioma:Inglês
Publicat: American Institute of Physics 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7028306/
https://ncbi.nlm.nih.gov/pubmed/32095020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4830046
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