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Ultra-high throughput detection of single cell β-galactosidase activity in droplets using micro-optical lens array
We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli β-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their...
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| Publicado no: | Appl Phys Lett |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Institute of Physics
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7028306/ https://ncbi.nlm.nih.gov/pubmed/32095020 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4830046 |
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