Ultra-high throughput detection of single cell β-galactosidase activity in droplets using micro-optical lens array

We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli β-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their...

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Bibliographic Details
Published in:Appl Phys Lett
Main Authors: Lim, Jiseok, Vrignon, Jérémy, Gruner, Philipp, Karamitros, Christos S., Konrad, Manfred, Baret, Jean-Christophe
Format: Artigo
Language:Inglês
Published: American Institute of Physics 2013
Subjects:
Biophysics and Bio-Inspired Systems
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7028306/
https://ncbi.nlm.nih.gov/pubmed/32095020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4830046
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