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CRISPR mediated genome editing of the human fungal pathogen Candida albicans
This method describes the efficient CRISPR mediated genome editing of the diploid human fungal pathogen Candida albicans. CRISPR mediated genome editing in C. albicans requires Cas9, guide RNA, and repair template. A plasmid expressing a yeast codon optimized Cas9 (CaCas9) has been generated. Guide...
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| Gepubliceerd in: | J Vis Exp |
|---|---|
| Hoofdauteurs: | , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2018
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7020622/ https://ncbi.nlm.nih.gov/pubmed/30507925 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58764 |
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