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SpRY Cas9 Can Utilize a Variety of Protospacer Adjacent Motif Site Sequences To Edit the Candida albicans Genome

Candida albicans is a human fungal pathogen capable of causing life-threatening infections. The ability to edit the C. albicans genome using CRISPR/Cas9 is an important tool investigators can leverage in their search for novel therapeutic targets. However, wild-type Cas9 requires an NGG protospacer...

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Detalhes bibliográficos
Publicado no:mSphere
Main Authors: Evans, Ben A., Bernstein, Douglas A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8265644/
https://ncbi.nlm.nih.gov/pubmed/34011687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00303-21
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